PGC-based genome editing has established itself as a robust and versatile platform in poultry science. This review chronicles the technological evolution from early untargeted …
We developed a biosensor using Pyrococcus furiosus Argonaute (PfAgo) for the highly sensitive and specific detection of single-nucleotide mutations causing fungicide resistance. …
Using CUT&Tag sequencing, we mapped the genome-wide binding sites of the transcription factor MEF2A in chicken primary myoblasts, identifying 1,768 target genes involved in …
We investigated the tissue expression and functional characteristics of the calcitonin (CT) family peptides and their receptors in chickens. Our findings reveal distinct …
In conclusion, we cloned the full-length sequence of chicken MC5R gene and characterized its promoter activity in DF1 chicken embryonic fibroblasts. The functional assay …
In summary, we cloned NPS and NPSR1 genes from the chicken brain. Functional assays proved the cNPSR1 can be potently activated by its endogenous ligand NPS and trigger multiple …
In summary, the full-length cDNA of the three chicken 5-HT receptor genes (HTR1B, HTR1E, and HTR1F) were first reported in the present study. Sequence alignment showed that the …
In summary, our data sets a critical reference point to reveal the novel aspects of pituitary biology across vertebrates, and helps to figure out how the anterior pituitary, as a …
In addition, we found that pig MRAP2 can decrease surface expression, increase the ligand sensitivity and selectivity of pMC4RAsp/pMC4RAsn, and enhance the basal constitutive …
In summary, we cloned six genes of the MC4R signaling system in a representative teleost species, Nile tilapia. Functional study proved that MC4R can interact with MRAP2b and …
In summary, we proved that AVT possesses four functional receptors (AVPR1A, AVPR1B, AVPR2b and MTR), and MT has a single receptor (MTR).
In summary, we characterized two APLNRs in chickens and turtles, and three APLNRs in zebrafish.
The orphan receptor GPR25 was cloned from zebrafish, pigeons and spotted gars. GPR25 is activated by Apelin and Apela in non-mammalian vertebrates. Like Apelin receptor, GPR25 is …
Collectively, our data helps to elucidate the physiological roles of NMU/NMS peptides in birds and reveal the functional conservation and changes of NMU/NMS-NMUR axis across …
In summary, we revealed an intricate interaction network of MC4R/MC3R with α-MSH/ACTH, AgRP and MRAP2 (/MRAP) in chickens.
Taken together, our study offers novel information about the expression and functionality of cY1, cY4, cY6 and their ligands in birds, and helps to decipher their conserved roles …
On the whole, our data establishes a molecular basis to elucidate the actions of three functional NPY receptors (cY2, cY5 and cY7) and their ligands in birds, which helps to …
Taken together, our findings raise a novel concept that the actions of SCFAs are likely mediated by 2 FFA2s (FFA2 and FFA2L) in target tissues of some mammalian species, such as …